. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 252 GREGOR T. POPA. urchin eggs have been for a short time. The reaction is in- stantaneous. The spermatozoa exhibit great activity and bunch together, for a while, in compact clusters of various sizes. What is the immediate cause of the agglutination residing in the spermatozoa? Is it due to a change in the arrangement of the substances, or to an active elimination of the sticky substance observed in fresh suspensions of spermatozoa?. FIG. 10. Arrangement of agglutination experiment; a, egg-water, b, clusters- c, sperm-susp
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 252 GREGOR T. POPA. urchin eggs have been for a short time. The reaction is in- stantaneous. The spermatozoa exhibit great activity and bunch together, for a while, in compact clusters of various sizes. What is the immediate cause of the agglutination residing in the spermatozoa? Is it due to a change in the arrangement of the substances, or to an active elimination of the sticky substance observed in fresh suspensions of spermatozoa?. FIG. 10. Arrangement of agglutination experiment; a, egg-water, b, clusters- c, sperm-suspension, d, drop of Janus green. One drop of egg-water and one drop of sperm suspension are placed on a slide (Fig. 10). A very narrow bridge is established between the two drops. Immediately the agglutination takes place where the drops meet, and slowly spreads, so that we have again various stages of a phenomenon on the same slide. At the left end of the egg-water drop (Fig. 10) the fluid is still clear; at the middle, agglutinated clusters of spermatozoa in great activity; at the right side of the sperm suspension drop, the spermatozoa in various degrees of motion, are in a homo- geneous suspension. In these conditions, one keeps the slides various lengths of time (i-io minutes). Afterwards one puts very carefully a cover slip with another drop of egg-water underneath (to mitigate the power of capillarity). Many bunches of spermatozoa are spread out, but many other resist, and one can observe, under the oil immersion lens, what happens in the clusters. A long series of transformations of the individual spermatozoa occur, represented by the next sketch (Fig. n). These trans- formations take place under the influence of egg-water only. But in order to see them more clearly, one may add at the margin of the cover slip a drop of I per cent. Janus green in. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability -
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
