. Bonner zoologische Beiträge : Herausgeber: Zoologisches Forschungsinstitut und Museum Alexander Koenig, Bonn. Biology; Zoology. 186 Sven Affeld et al.: Homarine in Opisthobranchia. PPM 8 6 8 2 8 0 7,8 7 6 7 4 7 2 7 0 6,8 6 6 6 0 5 8 5 4 4 6 Fig. 2. 'H NMR spectrum ofpuritled homarine in mcthanol-d4. 300 MHz. and ehemical structure of homarine. The epithelium of Cuthoiui caenilca showed very few glandular cells, these were usually filled with dark stain- ing granular substances. 4. DISCUSSION Secondary metabolites clearly seem to play a role in the biotic inter


. Bonner zoologische Beiträge : Herausgeber: Zoologisches Forschungsinstitut und Museum Alexander Koenig, Bonn. Biology; Zoology. 186 Sven Affeld et al.: Homarine in Opisthobranchia. PPM 8 6 8 2 8 0 7,8 7 6 7 4 7 2 7 0 6,8 6 6 6 0 5 8 5 4 4 6 Fig. 2. 'H NMR spectrum ofpuritled homarine in mcthanol-d4. 300 MHz. and ehemical structure of homarine. The epithelium of Cuthoiui caenilca showed very few glandular cells, these were usually filled with dark stain- ing granular substances. 4. DISCUSSION Secondary metabolites clearly seem to play a role in the biotic interactions with potential predators throughout all systematic groups of Opisthobranchia. These chemicals are often taken up from the food while de novo synthesis is rare (review see Cimino et al. 2004). Only a few field studies on the deteiTcnt effect of the ob- served compounds have been cairied out (Thompson 1960; Avila & Paul 1997; Slattery et al. 1998; John- son & Willows 1999; Marín et al. 1999; Avila et al. 2000; Becerro et al. 2001; Iken et al. 2002; Rogers et al. 2002; Penney 2004). While even a few studies focus on parasites on opisthobranchs (Arnaud 1978; Ho 1981; Carefoot 1987; Jensen 1987; Huys 2001; Schrödl 2002, 2003), the effect of secondai7 metabolites on pathogens is hardly investigated. Teeyapant et al. (1993a, b) studied the uptake of secondai^y metabolites in Tylo- diiui perversa (Tylodinoidea) from its food sponge Aplysiiui {Veivngia) aerop/ioba and the antimicrobial ac- tivity of the sponge metabolites. However they only found antimicrobial activity in compounds that were not pres- ent in Tylodiiui perversa. Incorporation of homarine is shown here for slugs feed- ing on Hydrozoa as well as on the gorgonian octocoral E. singularis. The examined Hydrozoa contained homarine. which is in accord with its role as a moiphogen. suppos- edly in all hydrozoans (Berking 1986, 1987). The exam- ined gorgonian E. singiilaris also contained homarine as described


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