Clinical diagnosis : the bacteriological, chemical, and microscopical evidence of disease . by means of alkaline hypo-bromites, and the nitrogen given off as a gas is collected, whilst thecarbonic acid combines with caustic soda present. The apparatusrequired is represented in the accompanying figure. It consists of acylinder of stout glass of 100 cc. contents (b), expanding at its middle,and connected below by means of a binder and tight-fitting tap with asmaller tube (a), also of glass, which holds about 5 cc. It is importantthat the capacity of the latter, which serves to receive the urine,
Clinical diagnosis : the bacteriological, chemical, and microscopical evidence of disease . by means of alkaline hypo-bromites, and the nitrogen given off as a gas is collected, whilst thecarbonic acid combines with caustic soda present. The apparatusrequired is represented in the accompanying figure. It consists of acylinder of stout glass of 100 cc. contents (b), expanding at its middle,and connected below by means of a binder and tight-fitting tap with asmaller tube (a), also of glass, which holds about 5 cc. It is importantthat the capacity of the latter, which serves to receive the urine,together with that of the perforation in the tap, shall be accuratelyknown. To this end the apparatus is carefully washed with water, 364 THE TJKINE rinsed out with alcohol, and dried. The tap is opened, and mercurypoured into the lower vessel so as to overflow into the upper one. Thetap is now closed, the overflow of mercury ahove it poured off, and thecontents of a removed and weighed in a vessel whose own weight isknown. The result divided by the sp. gr. of mercury () gives the. Fig. 140.—Hiifner s Apparatus for Estimation of Urea. cubic contents of the tube a. This must be verified by repeating theprocess, and if the capacity ascertained should vary each time, the meanmust be taken. It should be calculated to three places of decimals. In cases where the method of weighing cannot be conveniently carried out,the capacity of the vessel destined to hold the urine may be ascertained withgreat accuracy thus:—This portion of the apparatus is filled with a watery solu- ESTIMATION OF UREA 365 tion of some aniline dye which is not taken up by chloroform, the apparatuswashed out with chloroform, and the tinted water together with the chloroform,in which little or none of the dye dissolves, is placed in an accurately graduatedburette. The chloroform is allowed to settle and the quantity of the anilinefluid in the burette is read off. This process should be gone through at leas
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Keywords: ., boo, bookcentury1800, bookdecade1890, booksubjectclinicalmedicine
