. Biological stains, a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy). CH, + = H,N_ H,N_/ fuchsin. XH3CI +0 XH. leuco-fuchsin Ordinarily this reaction is reversable under conditions favoring oxidation. It is of especial significance to the bacteriologist, as dj^es can often be used as indicators of reduction. Certain dyes form a still different type of leuco compound, often called a "; We have seen that the basic dyes ordinarily occur as salts of some colorless acid; now, in the case of certain d
. Biological stains, a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy). CH, + = H,N_ H,N_/ fuchsin. XH3CI +0 XH. leuco-fuchsin Ordinarily this reaction is reversable under conditions favoring oxidation. It is of especial significance to the bacteriologist, as dj^es can often be used as indicators of reduction. Certain dyes form a still different type of leuco compound, often called a "; We have seen that the basic dyes ordinarily occur as salts of some colorless acid; now, in the case of certain dyes, notably the tri-phenyl methanes and xanthenes (Chapters VI and VII), as soon as the acid radical is removed, the compound becomes colorless. This is because a rearrangement of the atoms in the molecule takes place upon neutralization so as to give, not the true dye base, but a compound known as a carbinol (see p. 59) in which the chromophore does not occur. Thus the theoretical base of fuchsin which should be obtained upon removal of the acid radical is: CH3 \ C=/ The compound actually formed, however, is the pseudo-base or carbinol:. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975; Ambler, Joseph Alfred, 1889-; Kornhauser, S. I. (Sidney Isaac), 1887-1959; Mallory, Frank Burr, 1862-1941; Sharp, Lester W. (Lester Whyland), b. 1887. Geneva, N. Y. , The Commission
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