. dan bai zhi jie gou fen xi zhi bei jian ding yu wei liang ce xu. botany. æ³çèåï¼å ¶ç®çæ¯ä¸ºäºè·å¾è¶³å¤å¤çé«äº§çå¤è½ä»èå¯è½ç¨äºä¸çº§ç»æçåæå·¥ä½ã é解æ¹å¼çéæ©ä¸»è¦åå³äºæéè¦ç段çæ°éå大å°ï¼Matsudaira 1993)ï¼å¨ä½å«é æ®åºï¼å¦Metï¼ Asp-ProæTip)çä½ç½®ä¸è¿è¡åå-¦é解æ¶ï¼å èµ°SDS-PAGE (Tricine-è¶ï¼ Schagger and von Jagow 1987),ç¶åå°åä¸è½ç带çµè½¬å°æ°æ§èä¸ï¼èäºä¹ç¨ï¼PVDF), å¯è½æ¯é²æ-¢å¾®éèç½æ ·åæ失çæä½³éæ©æ¹æ³ï¼ç¸åå°ï¼å°éæ ·åå¨é«åºç°é¢çæ®åº (ä¾å¦LysæAi?)çä½ç½®ä¸è¿è¡èç½é ¶è§£åç产ç©åæ好æ¯éç¨ç»çåç¸HPLCæ±è¿è¡ å离ã åå-¦åé ¶è§£è¯å æ常ç¨çåå-¦æ¹æ³æ¯ç¨CNBrç70%ç²é ¸æä¸æ°ä¹é ¸æº¶æ¶²å¨ç²ç¡«æ°£é ¸ç羧端å¤å
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