. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 608 KENNETH R. H. READ UJ LU Q o i— CL o PHACOIDES PECTINATUS HEMOGLOBIN i r. _L I I I I I I I I I I 600 400 500 WAVELENGTH nyi FIGURE 2. Absorption spectra of Pliacoidcs pectinatns hemoglobin and derivatives. included the fixation of the tissue in 10% acrolein in xylene at 0° C., embedding in wax and staining for occult iron by the methods of Click and Hutchison as described in Humason (1962), somewhat modified. The most satisfactory method for the iron stain proved to be the
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 608 KENNETH R. H. READ UJ LU Q o i— CL o PHACOIDES PECTINATUS HEMOGLOBIN i r. _L I I I I I I I I I I 600 400 500 WAVELENGTH nyi FIGURE 2. Absorption spectra of Pliacoidcs pectinatns hemoglobin and derivatives. included the fixation of the tissue in 10% acrolein in xylene at 0° C., embedding in wax and staining for occult iron by the methods of Click and Hutchison as described in Humason (1962), somewhat modified. The most satisfactory method for the iron stain proved to be the following: (1) Deceration and hydration of the tissue to water; (2) two minutes in 30% H2O2 alkalized with one drop concentrated NH3 solution per 100 ml.; (3) thor- ough rinsing with several changes of distilled water for 5 minutes; (4) 15 minutes at 56° C. in a freshly made, filtered and heated solution of acidified potassium ferrocyanide, made by mixing 25 ml. each of solutions containing 2 g. potassium ferrocyanide to 50 ml. water and 2 ml. concentrated HC1 to 50 ml. water; (5) thor- ough rinsing with several changes of distilled water for 5 minutes; (6) counter- staining 4 minutes in \% carminic acid; (7) 5 minutes' differentiation in 4% potassium aluminum sulfate; (8) thorough rinsing in several changes of distilled water for 5 minutes; (9) dehydration, clearing and mounting. The iron-containing material is stained a bright green and the nuclei red; the remainder of the tissues stain various shades of pink. RESULTS Absorption spectra The results of the measurement of the absorption spectra of the hemoglobin of Phacoides pectinotus are shown in Figure 2. In the following description the numbers in parentheses represent the range of the observations in m/* and the number of observations, respectively. The Pliacoidcs pectinatns preparation in. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - color
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
