. A laboratory manual of soil bacteriology. Soil microbiology. 20 SOIL BACTERIOLOGY to 40° C, to each plate. A blank plate or control should be poured with each series. In case the medium is turbid, heat slowly, allowing the deposit to settle. Use only the clear portion of the medium for pouring plates. 8. Immediately after adding the culture-medium rotate each plate to secure a uniform mixture. Allow agar plates to harden on a level Fig. I.—Agar plate showing a common form of spreading colonies found in soil. 9. Agar plates should be inverted and incubated under a moist chamber at 2
. A laboratory manual of soil bacteriology. Soil microbiology. 20 SOIL BACTERIOLOGY to 40° C, to each plate. A blank plate or control should be poured with each series. In case the medium is turbid, heat slowly, allowing the deposit to settle. Use only the clear portion of the medium for pouring plates. 8. Immediately after adding the culture-medium rotate each plate to secure a uniform mixture. Allow agar plates to harden on a level Fig. I.—Agar plate showing a common form of spreading colonies found in soil. 9. Agar plates should be inverted and incubated under a moist chamber at 28° C. The time of incubation will depend upon the different culture-media. After five to ten days count the number of colonies on each plate. If the colonies are not too thick, it is well to dot each one with a pen. When the colonies are too thick to count easily, use a hand lens and counting Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Fred, Edwin Broun, 1887-1981. Philadelphia, London, W. B. Saunders Company
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