. Botanical microtechnique. Botany -- Anatomy; Botany -- Morphology; Microscopy -- Technique; Microscopes -- Technique; Botany; Plants -- anatomy & histology; Microscopy. 'f^B^'*' mP^ 0. Fig. —Portion of pustule of Cystopus candidus on stem of Capsclla, Ciaf , acetone-tertiary butyl alcohol. Safranin-fast green and hemalum-safranin are both excellent combinations for elementary use, and iron hematoxylin is a good nuclear stain (Fig. ). Mature oospores are abundant in the older, hypertrophied stems and fruits of the host, therefore, a more vigorous kiUing Ikiid is need
. Botanical microtechnique. Botany -- Anatomy; Botany -- Morphology; Microscopy -- Technique; Microscopes -- Technique; Botany; Plants -- anatomy & histology; Microscopy. 'f^B^'*' mP^ 0. Fig. —Portion of pustule of Cystopus candidus on stem of Capsclla, Ciaf , acetone-tertiary butyl alcohol. Safranin-fast green and hemalum-safranin are both excellent combinations for elementary use, and iron hematoxylin is a good nuclear stain (Fig. ). Mature oospores are abundant in the older, hypertrophied stems and fruits of the host, therefore, a more vigorous kiUing Ikiid is needed; FAA and standard Nawaschin are both satisfactory. As might be expected, the host cells are in a distorted condition at this stage. Peronospora parasitica, a common parasite on crucifers, yields excellent preparations by the methods described for Albugo. The best compromise fluid for preserving both the host cells and the fungus is Craf II. Safranin-fast green differentiates the nuclei of the fungus, but not so sharply as iron hematoxylin. ASCOMYCETES The mold members of this group, such as Aspergillus and Penicillium, are so easy to culture and study from wet mounts that permanent slides are seldom necessary. Permanent slides can be made bv the common whole-mount methods. Such slides are useful for. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Sass, John E. (John Eugene), 1897-1971. Ames, Iowa State College Press
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Keywords: ., bookcentury1900, booksu, booksubjectbotany, booksubjectmicroscopy
