. The Biochemical journal. Biochemistry. THE ESTIMATION OF SUGAR IX BLOOD 151 readings falling sharphj with prolongation of the heating, and the sedimented corpuscles give even higher readings at the outset with a similar rapid fall. The only possible explanation of this result is the presence of a substance diflFering from glucose and mainly concentrated in the corpuscles which reacts with picric acid solution more rapidly than sugar, and by its additive effect leads to the ultimate high readings obtained by the Benedict method. This substance is present to a small extent only in the plasma,
. The Biochemical journal. Biochemistry. THE ESTIMATION OF SUGAR IX BLOOD 151 readings falling sharphj with prolongation of the heating, and the sedimented corpuscles give even higher readings at the outset with a similar rapid fall. The only possible explanation of this result is the presence of a substance diflFering from glucose and mainly concentrated in the corpuscles which reacts with picric acid solution more rapidly than sugar, and by its additive effect leads to the ultimate high readings obtained by the Benedict method. This substance is present to a small extent only in the plasma, and consequently estimations carried out on plasma by the Benedict method in normal bloods show only slight excess over those obtained by the method of MacLean. In hyperglycaemic bloods even the results obtained from plasma are likely to be unsatisfactory, owing to the lack of direct proportion between the intensity of colour produced and sugar present under the conditions of the 5 6 7 8 Duration of heating at 100' in minutes Fijr. 1 A glucose standard. B corpuscles C whole blood. D plasma. Conclusions. 1. The Benedict method for the estimation of sugar in blood gives results which are too high and shows an average figure about 30 to 50 % in excess of that found by MacLean's method. '2. The high results appear to be chiefly due to the presence of an inter- fering substance or substances mainly concentrated in the corpuscles but present to some extent in the plasma also; this substance reacts with the picric solution at an early stage of the heating. Creatinine probably plays a large part in this reaction. Bioch. xin 11. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Biochemical Society (Great Britain); University of Liverpool. Biochemical Dept. London [etc. ] Cambridge University Press
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