. Pathological technique; a practical manual for workers in pathological histology and bacteriology. bydirect extension from a tuberculous focus in the lung) oftenshows a large number or even a predominance of poly-nuclear leucocytes, owing to a secondary infection withpyogenic bacteria. Old mechanical effusions are occasionally encounteredwhere little endothelium is seen, and the cells found arealmost exclusively lymphocytes. Method.—The fluid should be drawn with the usualaseptic precautions into sterilized flasks or tubes. If it isclotted, it should be shaken until the clot is thoroughly co
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. bydirect extension from a tuberculous focus in the lung) oftenshows a large number or even a predominance of poly-nuclear leucocytes, owing to a secondary infection withpyogenic bacteria. Old mechanical effusions are occasionally encounteredwhere little endothelium is seen, and the cells found arealmost exclusively lymphocytes. Method.—The fluid should be drawn with the usualaseptic precautions into sterilized flasks or tubes. If it isclotted, it should be shaken until the clot is thoroughly con-tracted, and the clot, or all clots of large size, should beremoved. CLINICAL PATHOLOGY. 459 Place the fluid in centrifuge tubes and centrifugalize forfive minutes at least. Decant the supernatant fluid gentlyat first, and when asmall amount only remains, invert the tube for about twoseconds. A few drops only will be left. With a small platinum loop stir the sediment thoroughly,rubbing the sides of the glass to remove adherent the sediment is thoroughly mixed with the few drops. Fig. 149.—Cytodiagnosis. Endothelial cells in a smear preparation fromthe centrifugalized sediment of a transudate or mechanical effusion (Percy Mus-grave; photo by L. S. Brown). of fluid remaining after decantation, remove a drop of themixture with the platinum loop and make a cover-slip this to dry spontaneously or by very gently at the boiling-point will spoil the preparation. Cover the preparation with a staining fluid made as fol-lows : Wrights blood-stain, 3 parts; Pure methyl-alcohol, I part. Allow to remain on the preparation twenty to forty-fiveseconds, then dilute it with 8 to 10 drops of water and allowthis mixture to stand one to two minutes. Wash very gently, preferably by flooding the slide with adropper. Do this four or five times, allowing the water toremain on the slide a few seconds each time. Vigorous or 460 PATHOLOGICAL TECHNIQUE. forci
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