. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 334 T. ITOW AND K. SEKIGUCHI (mm) X »— o z LU r~ -2 -1 1 (days) FIGURE 7. Elongation of the embryonic area observed by cinemicrography in normal and treated embryos. Closed circle : length of embryonic area in normal embryos. Open circle : length of embryonic area in an embryo treated with dithiothreitol for 24 hr at the period shown. The treated embryo developed lacking the 3rd prosomal appendage in the right side and with fused 3rd and the 4th appendages on the left. Length of embryonic area was measured between
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 334 T. ITOW AND K. SEKIGUCHI (mm) X »— o z LU r~ -2 -1 1 (days) FIGURE 7. Elongation of the embryonic area observed by cinemicrography in normal and treated embryos. Closed circle : length of embryonic area in normal embryos. Open circle : length of embryonic area in an embryo treated with dithiothreitol for 24 hr at the period shown. The treated embryo developed lacking the 3rd prosomal appendage in the right side and with fused 3rd and the 4th appendages on the left. Length of embryonic area was measured between the anterior end and the blastopore. nucleoli differed between these two treatments. The number of nucleoli decreased with treatment by chemical reagents, but not with culture at 5° C. When embryos were treated with chemical reagents, no enucleated or polynucleated cell was observed. By electron microscopic observations, a difference in the adhesion of surface cells was recognized between normal embryos and embryos treated with chemical reagents. In normal embryos, the cells showed complete adhesion. However, in embryos treated with DTT or Cyt. B, adjacent cells were partly separated. Micro- filaments were observed at the apical circumferences of the cylindrical surface cells in the normal embryo, but they could not be observed in the cubic surface cells of the normal embryo or in the cubic cells whose transformation into cylindrical shape was stopped with chemical reagents. On the other hand, microtubules were observed even in the treated embryos. In embryos treated with chemical reagents, secretion of the inner egg membrane and transformation of the surface cells were stopped in the same state as before treatment. Organelles such as mitochondrion, Golgi body, ribosome, nucleus and cell membrane showed no recognizable difference between the normal and the treated embryos. By histological observations, no appreciable difference in histological features was recognized betwe
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
