Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954 electrolytesinbi00shan Year: 1955 DEAN B. COWIE AND RICHARD B. ROBERTS 3 of cells and then decanting without disturbing the cell pellet. Aliquots of the cells and supernatant fluid were taken for radioactivity measurements. The time elapsed between the immersion of the cells and the decanting of the supernatant fluid was 8 minutes. In table i the ratio, radioactivity per ml of cells/radioactivity per ml of medium, is shown to
Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954 electrolytesinbi00shan Year: 1955 DEAN B. COWIE AND RICHARD B. ROBERTS 3 of cells and then decanting without disturbing the cell pellet. Aliquots of the cells and supernatant fluid were taken for radioactivity measurements. The time elapsed between the immersion of the cells and the decanting of the supernatant fluid was 8 minutes. In table i the ratio, radioactivity per ml of cells/radioactivity per ml of medium, is shown to be independent of the sulfate concentration in the medium. 900 12 3 4 Fig. I. Fluid content of E. coli. This result demonstrates that a fixed relation exists between the sulfate within the cell and the sulfate of the medium. It is evident that within the 8-minute period of immersion and centrifugation an equilibrium is established across the cell membrane and that in these experiments approximately 72 of the total cell volume is available for the immediate uptake of sulfur. A similar result is obtained when cells are studied in cold saline solution in order to prevent possible metabolic uptake. Fluid Content of E. Coli. It can be shown that there is a close correlation between the water space volume of the cells and their actual fluid content.
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