Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . hooked or knobbed expansions. The largest transverse diameter is , in length the mycelium presents the greatest variations. (6). Blastomycetes. These organisms may be found in the cutaneous eruptions of the skinin blastomycosis and may be described as follows, according to Montgomeryand Ormsby. In unstained preparation the organisms appear as round or ovalbodies with a double-contoured highly refractive capsule. Within the capsule,in many instances, granules or spore-like bo
Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . hooked or knobbed expansions. The largest transverse diameter is , in length the mycelium presents the greatest variations. (6). Blastomycetes. These organisms may be found in the cutaneous eruptions of the skinin blastomycosis and may be described as follows, according to Montgomeryand Ormsby. In unstained preparation the organisms appear as round or ovalbodies with a double-contoured highly refractive capsule. Within the capsule,in many instances, granules or spore-like bodies can be distinguished. The i6o DIAGNOSTIC METHODS. addition of a i to lo per cent, solution of potassium hydrate to the specimenunder examination facilitates the recognition of these bodies. In stainedsections the double contoured, homogeneous capsule is usually separated froma finely or coarsely granular protoplasm by a clear space of varying of different sizes are found in some organisms. In both pus and tissue,organisms in pairs or in various stages of budding are commonly seen. The. Fig. 68.—Mycelial threads of blastomyces from old agar culture.(From photograph by W. A. Pusey.) parasite, as a rule, varies in size from 7 to 20 microns, though slightly smallerand much larger forms occur in some cases. The organisms are readily obtained in pure culture from unbroken ab-scesses, from miliary abscesses in the borders of the cutaneous lesions, and fromthe miliary nodules and abscesses in the deep-seated organs. The peculiaritiesof the cultures of blastomycetes must be looked for in other works. Micro- PARASITES. l6l scropically, the organism obtained in culture appears at first as a fine, branchingmycelium with a few small spore-like bodies. Later a large, segmented, oftenpod-like mycelium appears, together with large, round, or oval bodies withbud-like projections. BIBLIOGRAPHY. 1. Carpenter. The Microscope and its Revelations. Philadelphia, 1901. 2. Daniels
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