. Biological structure and function; proceedings. Biochemistry; Cytology. CELL DIVISION AND PROTEIN SYNTHESIS 54I offered at time EH,* regularly dropping precentages when the compound is added later, finally almost no labelling when cells in maximal division i are exposed. This can be repeated with "washed cells" (defined in Section 2). Immersion of washed synchronized cells into a solution of 5-fluoro-2-deoxyuridine (5 x 10 -^ m) at EH, thus prior to the addition of the tritiated thymidine reduces to 6-12"',, the average number of tracks over the nucleus which takes the label.
. Biological structure and function; proceedings. Biochemistry; Cytology. CELL DIVISION AND PROTEIN SYNTHESIS 54I offered at time EH,* regularly dropping precentages when the compound is added later, finally almost no labelling when cells in maximal division i are exposed. This can be repeated with "washed cells" (defined in Section 2). Immersion of washed synchronized cells into a solution of 5-fluoro-2-deoxyuridine (5 x 10 -^ m) at EH, thus prior to the addition of the tritiated thymidine reduces to 6-12"',, the average number of tracks over the nucleus which takes the label. It does not significantlv alter the percentage of labelled cells. This is so for all time points studied. The long exposures to this base analogue interfere neither with division i nor with. OS Fk;. 3. The open circles represent log cell number per ml. culnire. The filled circles represent DXA (microbiological assays) and the crosses ( x ) packed cell volume per ml. culture. Increases in cell volume and in DXA go parallel, for the culture and for the average cell. Plotted from Table i in Zeuthen and Scherbauni [14]). dixision 2. It is not vet proven, but it can be suggested that two syn- chronous divisions can be developed without (^e novo synthesis of DXA after the termination of the temperature shocks. 4. Studies with amino acid analogues The ideas developed in Section 2 have stood the first test with amino acid analogues. The experiments were performed in association with cand. mag. Leif Rasmussen. DL-/)-fluorophenylalanine (/)-FPhe) is a strong inhibitor of cell division * The time when the last temperature shock ends, as shown by a signal on the control Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry;
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