. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 5. Phase microscopy of paniculate tubulin fractions in extracts of oocytes of S[>i.\it/a. Unactivated oocytes (A), were homogenized and centrifuged as described in the Materials and Methods to yield a 2500 g supernatant (B) and 2500g pellet that contains the interphase paniculate tubulin (C-F). Tandemly arranged large spheres (large arrows) and small panicles (small arrows) are absent from the supernatant fractions. After centrifugation, the small particle is frequently lost and only the larger sphere is observed.
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 5. Phase microscopy of paniculate tubulin fractions in extracts of oocytes of S[>i.\it/a. Unactivated oocytes (A), were homogenized and centrifuged as described in the Materials and Methods to yield a 2500 g supernatant (B) and 2500g pellet that contains the interphase paniculate tubulin (C-F). Tandemly arranged large spheres (large arrows) and small panicles (small arrows) are absent from the supernatant fractions. After centrifugation, the small particle is frequently lost and only the larger sphere is observed. Membrane- like (M) structures are frequently associated with the larger sphere. Compare the morphology of the tandemly arranged particles to the nucleolus (N) and nucleolinus (L) of the intact oocyte (5A). Within the germinal vesicle (GV) lie a tandemly arranged structure indistinguishable from the structures in the 2500g pellet. Bar = 20 Mm. Figure 6. Hoechst DNA staining of the 2500g pellet fraction. Pellets were resuspended in HGL. incubated for 10 min on poly-L-lysine coated coverslips, rinsed twice with HGL and finally with HGL containing 2 Mg/ml Hoechst 22358. The magnification bar is 10 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
