. Bacteriology and the public health. Bacteriology; Public health. 314 BACTERIA AND DISEASE It is a non-liquefying organism, pathogenic for white mice (producing septicaemia). It grows on ordinary laboratory media, producing a viscid tenacious culture. 5. B. coli communis and many putrefactive germs commonly occur in suppurative conditions, but they are not restricted to such disorders (see p. 46). 6. Micrococcus gonorrhoeae (Neisser, 1879).—This organism is more frequently spoken of as a diplococcus. It occurs at the acute stage of the disease (and in the purulent secretion of gonorrheal conj
. Bacteriology and the public health. Bacteriology; Public health. 314 BACTERIA AND DISEASE It is a non-liquefying organism, pathogenic for white mice (producing septicaemia). It grows on ordinary laboratory media, producing a viscid tenacious culture. 5. B. coli communis and many putrefactive germs commonly occur in suppurative conditions, but they are not restricted to such disorders (see p. 46). 6. Micrococcus gonorrhoeae (Neisser, 1879).—This organism is more frequently spoken of as a diplococcus. It occurs at the acute stage of the disease (and in the purulent secretion of gonorrheal conjunctivitis), but is not readily differentiated from other similar diplococci except by laboratory methods. Each element of the diplo- coccus presents a straight or concave surface to its fellow. A very marked concavity indicates commencing fission. The position which these diplococci take up in pus is intracellular, and they are arranged more or less definitely around the nucleus. In chronic gonorrhoea the diplococci are diminished in number. Difficulty has often been found in culti- vating the organism in artificial media outside the body. Wertheim and others have suggested special formulae for the preparation of suitable media, but it is a comparatively simple matter to secure cultures on agar plates smeared with human blood from a pricked finger. The plate is incubated at 37° C. At the end of twenty-four hours small raised grey colonies appear, which at about the end of four days show adult growth. The margin is uneven, and the centre more opaque than the rest of the colony. This diplococcus is readily killed, and sub-cultures must be frequently made to retain vitality and virulence. Light, desiccation, and a temperature of 55° C. all act germicidally. The organism stains readily with Loffler's blue, but is decolorised by Gram's method. It is more or less strictly parasitic to man, and has been definitely proved to be the cause of gonorrhoea. A toxin has been separated.
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