. dai xie gong cheng. botany. å¯åçèµæº (çº¤ç»´ç´ ãæ·ç²ãä¹³ç³ï¼. è¡èç³ è¡èç³è±æ°¢é ¶ NADP* æ°§åé NADPH è¡èç³é ¸ å¾å¯åçèµæºè½¬å为å¤æ° è½ï¼å çµææ¯ï¼ãæ°ååè£è§£ç-ã ç®åï¼ä¹å¨æ¢ç´¢å©ç¨åé µæèææ¯ä½ä¸ºæ½å¨ç çç©è·¯çº¿ç产å¤æ°ï¼Kitani and Hall, 1989; Taguchi etal, 1995)(è§å¾)ãä¼æå¨ç¥ï¼ç-éçå¾®ç ç©å¯ä»¥å°æ°£æ°ä½ä¸ºä»£è°¢ç»äº§ç©èé«æç产ã代谢工 ç¨ææ¯å°è¡¨æï¼å¨æç»è代谢æ¹åé«äºççæ°´å¹³ç æ°£æ°ç产æ¹é¢è¿å°ååæç¨ãè³ä»ï¼å·²å离åºåä¸ æ°£åæçå 个åºå ï¼ä¾å¦åèªå¼æ°æ æª¬é ¸æè (Citrobacter freudii)çæ°£åèåºå å·²å¨Â£ . coliè ä¸-å éï¼Kanamayia et alï¼ 1988)ã æè¿ï¼å·²ç»ç 究äºä½å¤ç产氣æ°çå¯è½æ§ (Woodward et al,
. dai xie gong cheng. botany. å¯åçèµæº (çº¤ç»´ç´ ãæ·ç²ãä¹³ç³ï¼. è¡èç³ è¡èç³è±æ°¢é ¶ NADP* æ°§åé NADPH è¡èç³é ¸ å¾å¯åçèµæºè½¬å为å¤æ° è½ï¼å çµææ¯ï¼ãæ°ååè£è§£ç-ã ç®åï¼ä¹å¨æ¢ç´¢å©ç¨åé µæèææ¯ä½ä¸ºæ½å¨ç çç©è·¯çº¿ç产å¤æ°ï¼Kitani and Hall, 1989; Taguchi etal, 1995)(è§å¾)ãä¼æå¨ç¥ï¼ç-éçå¾®ç ç©å¯ä»¥å°æ°£æ°ä½ä¸ºä»£è°¢ç»äº§ç©èé«æç产ã代谢工 ç¨ææ¯å°è¡¨æï¼å¨æç»è代谢æ¹åé«äºççæ°´å¹³ç æ°£æ°ç产æ¹é¢è¿å°ååæç¨ãè³ä»ï¼å·²å离åºåä¸ æ°£åæçå 个åºå ï¼ä¾å¦åèªå¼æ°æ æª¬é ¸æè (Citrobacter freudii)çæ°£åèåºå å·²å¨Â£ . coliè ä¸-å éï¼Kanamayia et alï¼ 1988)ã æè¿ï¼å·²ç»ç 究äºä½å¤ç产氣æ°çå¯è½æ§ (Woodward et al, 1996)ãæ-¤ç³»ç»ç±ä¸¤ç§SIç»æï¼å³ ä»åé ¸ç-åä½(Thermoplasma acidophilum )ä¸-å 离å°çè¡èç³è±æ°¢é(GDH)åä»æ¿çç«çè iPyrococcus furiosus)ä¸-å离å°çæ°¢åè¤ãGDHå¬ åè¡èç³çæ°§åçæè¡ç³é ¸-<^å é ¯ï¼å ¶ç¨NADHæNADPHä½ä¸ºè¾ å©å å-è¿ä¸æ-¥æ°´è§£å½¢æ è¡èç³é ¸ã尽管ç»èæ°£åçç±äºä¸éå½çä½å¿è½æå°ä¸NADPHç¸äºä½ç¨ï¼ä½åèªæ¿çç« çèï¼P. furiosus)åçå »äº§ç¢±èï¼A. eutrophus)çæ°¢åè¤å·²è¡¨æå©ç¨NADPHä½ä¸ºä¸ 个çµå-ä¾ä½ãWoodwardç-ï¼1996)è¯å®GDHä¸æ°¢åéçç»åå ·æå¨ä½å¤ç±è¡èç³ç产氣 æ°çè½åï¼è§å¾)ãå¨è¾ å©å å-ä¸æ-循ç¯ï¼è³å°20次ï¼çæ åµä¸ï¼ç±è¡èç³ç产çæ°¢ æ°éå¯è¾¾å°åå-¦è®¡éå¾çãè¿æ¡æ°åç°çéå¾ä¼¼ä¹æ¯ä»å¯åçèµæºç产æ
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