. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 48 W. H. McSHAN, SOL KRAMER AND N. F. OLSON and Potter, 1943). The p,g. of phosphorus released in 15 minutes by the different amounts of tissue were used for calculating the //.g. of phosphorus hydrolyzed by 1 mg. of tissue. The values used for preparing the graphs and given in the tables are expressed in terms of the of phosphorus released by 1 mg. of muscle in 15 minutes. These values represent the number of ATPase units per mg. of muscle. RESULTS Relation of ATP concentration to enzyme activity Maximum ATPase activi
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 48 W. H. McSHAN, SOL KRAMER AND N. F. OLSON and Potter, 1943). The p,g. of phosphorus released in 15 minutes by the different amounts of tissue were used for calculating the //.g. of phosphorus hydrolyzed by 1 mg. of tissue. The values used for preparing the graphs and given in the tables are expressed in terms of the of phosphorus released by 1 mg. of muscle in 15 minutes. These values represent the number of ATPase units per mg. of muscle. RESULTS Relation of ATP concentration to enzyme activity Maximum ATPase activity was obtained when 2 mg. of muscle tissue were incubated with as low as X 10~3 M ATP. In order to insure an excess of substrate a final concentration of X 10~3 M ATP was used in the system for the routine determinations. oWITH Co*"1" • WITHOUT Co** o WITH Ccf* • WITHOUT Co+t FEMALE AMERICAN COCKROACH MALE AMERICAN COCKROACH. MG TISSUE MG TISSUE FIGURE 2. Effect of Ca ions on the release of inorganic phosphorus from ATP by the ATPase of muscle from the male and female cockroach. Effect of time and enzyme concentration The rate at which inorganic phosphorus was released from ATP by different amounts of tissue was not constant with time. This decrease in ATPase activity with time has been reported for liver homogenates (Novikoff et al., 1952), for purified yeast ATPase (Meyerhof and Ohlmeyer, 1952), and for mitochondria of flies (Sacktor, 1953). The results given graphically in Figure 1 for the muscle of the female cockroach and female woodroach show that the amounts of inorganic phosphorus released were linearly related to the enzyme concentration when , , and mg. of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. );
Size: 1928px × 1297px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
