. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 20- o- ^ - O •o B. 24 1 4 HOURS AFTER INJECTION 24 FIGURE 1. Concentration of total radiolabeled ecdysone and its metabolites following injection of [3H]ecdysone in (A) normal crabs in stage D,, and (B) LBA crabs in stage D,, (with partially regenerated limbs autotomized at t = 0 hr). The pattern of change is indicated for serum (closed boxes), epidermis (closed circles), hindgut (closed triangles), total crab (open boxes), midgut gland (open triangles), and carcass (open circles). As an estimate of the variability within ex
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 20- o- ^ - O •o B. 24 1 4 HOURS AFTER INJECTION 24 FIGURE 1. Concentration of total radiolabeled ecdysone and its metabolites following injection of [3H]ecdysone in (A) normal crabs in stage D,, and (B) LBA crabs in stage D,, (with partially regenerated limbs autotomized at t = 0 hr). The pattern of change is indicated for serum (closed boxes), epidermis (closed circles), hindgut (closed triangles), total crab (open boxes), midgut gland (open triangles), and carcass (open circles). As an estimate of the variability within experimental groups, the radioactivity in the serum of individual crabs was determined and results are plotted as the mean ± 1 SEM. RESULTS Metabolites of ecdysone [3H] Ecdysone was very rapidly converted by tissues of crabs in stage D0 to a total of five metabolites, two of which co-chromatographed with authentic 20- hydroxyecdysone and 20,26-dihydroxyecdysone (see Fig. 1 in McCarthy and Skinner, 1979). Three radiolabeled polar components were also observed. These components have been identified in intermolt crabs as polar conjugates, probably glycosides, of the three free ecdysteroids (McCarthy and Skinner, 1979). In the hindgut, a sixth radiolabeled metabolite was detected which was very polar and eluted from the reverse phase column in the void volume. A component with similar chromatographic properties was detected in the urine and feces of inter- molt crabs (McCarthy and Skinner, 1979) ; this unidentified metabolite probably represents an excretion product of ecdysteroids. Metabolic fate of ecdysone in various tissues The change in the total radioactivity (Figs. 1A, B) and the relative amounts of the several ecdysone metabolites (Tables I and II) were determined in several. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
