. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 688 S. J. O'DELL AND G. C. STEPHENS TABLE II Concentrations ofamino acids in the body wall o/Pareurythoe Amino acid Cone. (mM) Amino acid Cone. (mM) ASN ARC ALA VAL ASP ILE GLU GLN LYS THR LEU PHE MET TYR SER TAU GLY HIS Total amino acid concentration = mM. n = 3. supplied in the medium were reduced substantially below their initial concentration of 200 nM during the observations. To clarify the relationship betwee
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 688 S. J. O'DELL AND G. C. STEPHENS TABLE II Concentrations ofamino acids in the body wall o/Pareurythoe Amino acid Cone. (mM) Amino acid Cone. (mM) ASN ARC ALA VAL ASP ILE GLU GLN LYS THR LEU PHE MET TYR SER TAU GLY HIS Total amino acid concentration = mM. n = 3. supplied in the medium were reduced substantially below their initial concentration of 200 nM during the observations. To clarify the relationship between influx and the net entry of individual amino acids, simultaneous measurements of influx (disappearance of 14C-labeled amino acid from the medium) and net flux (decrease in concentration of the same amino acid as measured by HPLC) were performed. Such measurements were made for representative acidic (aspartate), basic (lysine), nonpolar neutral (alanine, glutamine), and polar neutral amino acids (serine, glycine). The initial concentration in the incu- bation medium (14C-labeled plus unlabeled) ranged from 300 to 400 nM depending on the specific activity of labeled compounds. Fluxes were measured for 90 min. The results shown in Figure 2 for serine are typical. In no case did least squares regression analysis of the influx and net flux data yield curves whose coefficients were signifi- cantly different. CONCENTRATION 400 - 300 OF SERINE IN THE MEDIUM 2QQ (nM) 100. 30 60 TIME (min) 90 Influx of labeled serine compared to net serine flux as measured by HPLC. Influx data are repvy diamonds (4). Net flux data are represented by circles (•). The exponential curve was fit to but1' :ts by least squares regression (r2 = ).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods
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