. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. DB LGC * Com • CG DB LGC. Figure 7. //; .S-//H hybridization with a 35S-labelled cDNA-probe in sections of the cerebral ganglia. Only hybridization of mRNA in the light green cells (LGC) and canopy cell (not shown) was observed. DB, dorsal body; CG, cerebral ganglia; Com, commissure. vous system-specific library ofLymnaea cloned in Xgt 10. Therefore, replica filters of 20,000 clones were screened with a positive cDNA probe synthesized from messenger RNA of the LGC, and with a negative cDNA probe pro- duced from other parts o
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. DB LGC * Com • CG DB LGC. Figure 7. //; .S-//H hybridization with a 35S-labelled cDNA-probe in sections of the cerebral ganglia. Only hybridization of mRNA in the light green cells (LGC) and canopy cell (not shown) was observed. DB, dorsal body; CG, cerebral ganglia; Com, commissure. vous system-specific library ofLymnaea cloned in Xgt 10. Therefore, replica filters of 20,000 clones were screened with a positive cDNA probe synthesized from messenger RNA of the LGC, and with a negative cDNA probe pro- duced from other parts of the cerebral ganglia and the hepatopancreas. The LGC specificity of the clones was tested by in situ hybridization using histological sections of the central nervous system (Fig. 7). The LGCs in the cerebral ganglia, and the canopy cell in each lateral lobe (not shown), were the only cell types to express this clone. The nucleotide sequence revealed a single open read- ing frame encoding a protein with characteristics of pre- proinsulin (Fig. 8) (Smit et ai. 1988). Thus, an A and B chain, together with a C peptide equivalent and a puta- tive signal sequence, are present. We called this peptide molluscan insulin-related peptide (MIP). Comparison of Invertebrate and Vertebrate Insulins Overall, the amino acid sequences of MIP, bombyxin, and human insulin, are not very similar (Fig. 9). The se- ! Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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