. The principles of bacteriology; a practical manual for students and physicians. Bacteriology. BACTERIUM INFLUENZA 401 should be pale red). (Fig. 76.) It is decolorized by the method of Gram. It develops only at temperatures ranging from 26° to 43° C. Its optimum temperature for growth is 37° C. It possesses the peculiarity of developing upon only those artificial culture-media to which blood or blood-coloring- matter has been added. Its cultivation is best conducted Fig. 76. Bacterium influenzse in sputum. and its development most satisfactorily observed by the following procedure: over the
. The principles of bacteriology; a practical manual for students and physicians. Bacteriology. BACTERIUM INFLUENZA 401 should be pale red). (Fig. 76.) It is decolorized by the method of Gram. It develops only at temperatures ranging from 26° to 43° C. Its optimum temperature for growth is 37° C. It possesses the peculiarity of developing upon only those artificial culture-media to which blood or blood-coloring- matter has been added. Its cultivation is best conducted Fig. 76. Bacterium influenzse in sputum. and its development most satisfactorily observed by the following procedure: over the surface of ,a slanted agar tube or over agar-agar solidified in a Petri dish smear a small quantity of sterile blood (not blood-serum). A bit of the mucus from the sputum of the influenza patient is then taken up with sterilized forceps or on a sterilized wire loop, rinsed in sterile bouillon or water and rubbed over the sur- face of the prepared agar-agar. The plate or tube is then 26 *. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Abbott, Alexander Crever, 1860-1935. Philadelphia, Lea & Febiger
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