. Bulletin of the United States Fish Commission. Fisheries -- United States; Fish-culture -- United States. EXPERIMENTAL STUDY OF THE OYSTER GILL II The large tank is filled with water up to the level of the vertical tube c. When equilibrium is established the rubber tube inserted into the oyster is connected to the horizontal tube b and the water from the gills begins to flow into the small vessel. The overflowing water is collected in a graduate. , The hydrostatic pressure inside the gill cavity can be measured by plugging the vertical tube c and watching, in the water gauge (g), the rise of
. Bulletin of the United States Fish Commission. Fisheries -- United States; Fish-culture -- United States. EXPERIMENTAL STUDY OF THE OYSTER GILL II The large tank is filled with water up to the level of the vertical tube c. When equilibrium is established the rubber tube inserted into the oyster is connected to the horizontal tube b and the water from the gills begins to flow into the small vessel. The overflowing water is collected in a graduate. , The hydrostatic pressure inside the gill cavity can be measured by plugging the vertical tube c and watching, in the water gauge (g), the rise of the level in the small vessel. In a few minutes a maximum difference is reached and the flow of water through the tube b ceases. This indicates that there is no more difference in pressure between the inside of the gill cavity and the end of the tube Fig. 2.—Tank for collecting the water after it passed through the gills, and measuring the hydrostatic pressure inside the gill cavity. 6.—tube coimecting the two vessels; c.—overflow; g.—water gauges; I.—constant level arrangement; p.—pipe supplying fresh sea water; (.—thermometer. .Approximately one-third natural size The difficulty in employing the tank method lies in the necessity of being very careful to keep the water in both vessels at a constant level. The rise of level in the large tank forces the water to flow by gravity through the gills, while a rise in the level in the small vessel retards the rate of flow because the gill epithelium is forced to work against the pressure. The method is indispensable, however, for collecting the water that had passed by the gills. CARMINE METHOD The rate of flow of water can be determined easily by the carmine method (Galt- sofl, 1928), which is as follows: The oyster is rigged up in the same manner as in the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these
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