. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 1. Ouchterlony agar diffusion tests. The center wells of all plates contain anti- Arbacia sperm serum No. 33. All surrounding wells were filled with extract and fluid of Ar- bacia punctulata. Each well received ml. of the sample: (a) (1 ) supernatant over frozen- thawed sperm after centrifugation at 26,000 X g for 20 minutes; (2) residue of No. (1), resuspended in sea water and centrifuged at low speed; (3) supernatant over Mickle-clisinte- grated sperm; (4) seminal plasma; (5) "frozen-thawed extract" of
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 1. Ouchterlony agar diffusion tests. The center wells of all plates contain anti- Arbacia sperm serum No. 33. All surrounding wells were filled with extract and fluid of Ar- bacia punctulata. Each well received ml. of the sample: (a) (1 ) supernatant over frozen- thawed sperm after centrifugation at 26,000 X g for 20 minutes; (2) residue of No. (1), resuspended in sea water and centrifuged at low speed; (3) supernatant over Mickle-clisinte- grated sperm; (4) seminal plasma; (5) "frozen-thawed extract" of sperm, low speed centri- fuged; (6) body fluid; (b) (1) seminal plasma; (2) "frozen-thawed extract" of whole sperm, low speed centrifugation; (3) "frozen-thawed extract" of whole sperm, low speed centrifuga- tion; (4) "frozen-thawed extract," low speed centrifugation; (5) supernatant over Mickle- disintegrated sperm, low speed centrifugation; (6) supernatant over Mickle-disintegrated sperm, low speed centrifugation; (c) (1) "frozen-thawed extract," low speed centrifugation; (2) basic protein, pH extract; (3) supernatant over washed sperm after standing (aging), low speed centrifugation; (4) supernatant over citric acid-extracted sperm, low speed centrifu- gation; (5) supernatant over urea-treated sperm; (6) frozen-thawed extract, low speed cen- trifugation; (d) (1) isolated heads, "frozen-thawed extract," low speed centrifugation; (2) isolated tails, "frozen-thawed extract," low speed centrifugation; (3) whole sperm, "frozen- thawed extract," low speed centrifugation; (4) isolated heads, "frozen-thawed extract," low speed centrifugation; (5) acid extract (pH 3) of sperm, low speed centrifugation; (6) acid extract (pH ) of sperm, low speed centrifugation. sperm. However, the one band that has been clearly demonstrated in the super- natant of aging sperm is band a of "frozen-thawed extrac
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
