. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. NUTRIENT MEDIA AND METHODS OF CULTIVATION. 107 vessel, and held in position by means of a clip. Tlie vessel can be fixed on the inclined stage of the microscope, and the contents of the tube conveniently examined with low-power objectives. Plate-cultivations.—By this method, as already mentioned, a mixture of bacteria, whether in fluids, excreta, or in cultivations on solid media, can be so treated that the different sp


. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. NUTRIENT MEDIA AND METHODS OF CULTIVATION. 107 vessel, and held in position by means of a clip. Tlie vessel can be fixed on the inclined stage of the microscope, and the contents of the tube conveniently examined with low-power objectives. Plate-cultivations.—By this method, as already mentioned, a mixture of bacteria, whether in fluids, excreta, or in cultivations on solid media, can be so treated that the different species are isolated one from the other, and perfectly pure cultivations of each of the cultivable bacteria in the original mixture established in various nutrient media. We are enabled also to examine under a low power of the microscope the individual colonies of bacteria, and to hj their characteristic appearances, micro-organisms which, in their individual foi-m, closely resemble one another, or ai'e even identical. The same process, with slight modification, is also employed in the examination of air, soil, and water, which will be referred to later. The preparation of plate-cultivations, therefore, must be described in every de- tail : and to take an example, we will sup- pose that a series of plates is to be pre- pared from a cube - cultivation. Arrangement of Levelling Fic. 36.—Levelling Appakatus, -In order to spread out the liquid jelly evenly on the surface of a glass plate, and hasten its sohdifica- tion, it is necessary to place the glass plate upon a level and cool surface. This is obtained in the following manner : Place a large shallow glass dish upon a tripod stand, and fill it to the brim with cold water; carefully cover the dish -vvith a slab of plate-glass, or a pane of window-glass, and level it by placing the i^pirit-level in the centre and adjusting the of the tripod. Substitute for the spirit-level a piece of fil


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Keywords: ., bookcentury1800, bookdecade1890, bookpublisherphila, bookyear1897