. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HOMOGENATE GLYCOLYSIS 261 H 0 40 80 120 FIGURE 5. Rates of lactic acid production by anaerobic homogenates. Clutch Solid line. A', pipiens. Broken line, hybrids. Substrate, glycogen. Abscissa, standard age in hours, 18° C. Ordinate, ,ug. lactic acid per embryo per hour, 24° C. In special experiments, we have found that a linear production of lactic acid commences within three minutes after the onset of gassing and persists for at least two hours. Our routine procedure, however, is implicit in the graph
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HOMOGENATE GLYCOLYSIS 261 H 0 40 80 120 FIGURE 5. Rates of lactic acid production by anaerobic homogenates. Clutch Solid line. A', pipiens. Broken line, hybrids. Substrate, glycogen. Abscissa, standard age in hours, 18° C. Ordinate, ,ug. lactic acid per embryo per hour, 24° C. In special experiments, we have found that a linear production of lactic acid commences within three minutes after the onset of gassing and persists for at least two hours. Our routine procedure, however, is implicit in the graphs of Figure 2, which also show how the factor of initial lactic acid was controlled. For each ex- periment, enough homogenate was prepared to load four flasks in the gassing train. At intervals, flasks were removed from the train and the amounts of lactic acid present were determined. A graph relating the amount of lactic acid present to the time spent under anaerobiosis was then prepared, and the slope of the line obtained was used to calculate the rate of glycolysis. Departures from the principles of this procedure will be mentioned explicitly in the appropriate contexts. All else being equal, generalization of the results obtained with the homogenate technique is most feasible when the activity being measured is a linear function of the amount of homogenized tissue present. Attempts to establish that this de- sideratum is satisfied by our own homogenate systems have not yielded altogether satisfactory results (Fig. 3) ; for, although the graphs relating the number of ho- mogenized embryos per ml. of homogenate to the glycolytic rate exhibited are linear, as we hoped, they do not pass through the origins. We have not been able to over- come this difficulty, and the reasons for it are not clear. Our other data, therefore, must be regarded as holding only for the standard homogenate concentration (10 embryos per ml.) that we have employed. Finally, we should mention that the
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
