. Biophysical science. Biophysics. 484 Absorption Spectrophotometry /26 : 2 standard spectrophotometers which increase their utility for biological research. It is quite possible to use a spectrophotometer without any idea of the physical basis for the observed spectra. However, spectrophotometry is so important in biological studies that it is hoped the reader will want to know more about the spectra in order to appreciate both the limitations and the unexplored possibilities of spectrophotometry. The physical basis for the characteristic absorption spectra of biological molecules is describe
. Biophysical science. Biophysics. 484 Absorption Spectrophotometry /26 : 2 standard spectrophotometers which increase their utility for biological research. It is quite possible to use a spectrophotometer without any idea of the physical basis for the observed spectra. However, spectrophotometry is so important in biological studies that it is hoped the reader will want to know more about the spectra in order to appreciate both the limitations and the unexplored possibilities of spectrophotometry. The physical basis for the characteristic absorption spectra of biological molecules is described in the following chapter. 2. Units and Symbols of Absorption Almost all biophysics, biochemistry, and physiology laboratories use some form of spectrophotometry. The most frequently used types are absorption spectra of compounds in liquid suspension or within the. 660 A (muJ Figure I. Absorption spectrum of ethyl chlorophyllide b. This spectrum is essentially identical to that for chlorophyll b. See Chapter 20 for a discussion of chlorophylls. After A. S. Holt and E. E. Jacobs, Am. J. Botany 41 : 710 (1954). living cell. These absorption spectra are used both to identify com- pounds and also to quantify the amount of a given compound in solution (or in the cell). Typical absorption spectra are shown in Figures 1 through Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ackerman, Eugene, 1920-. Englewood Cliffs, N. J. , Prentice-Hall
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