. The Biological bulletin. Biology; Zoology; Marine biology. 372 GREGORY A. AHEARN phorus by H. atra. The general procedure followed for reducing the holothurian bacterial population was adapted from that used by Rigler (1961) to reduce bac- terial flora on Daphnia magna. Six animals were held in sea water for three days prior to initiation of the experiment so that the gut contents might be eliminated. Ten liters of a pre-incubation medium were prepared by filtering sea water (HA O integument •O integument witti antibiotics. 0 6 12 18 24 30 36 42 48 54 ELAPSED TlME{tiours) Figure 3. The chang
. The Biological bulletin. Biology; Zoology; Marine biology. 372 GREGORY A. AHEARN phorus by H. atra. The general procedure followed for reducing the holothurian bacterial population was adapted from that used by Rigler (1961) to reduce bac- terial flora on Daphnia magna. Six animals were held in sea water for three days prior to initiation of the experiment so that the gut contents might be eliminated. Ten liters of a pre-incubation medium were prepared by filtering sea water (HA O integument •O integument witti antibiotics. 0 6 12 18 24 30 36 42 48 54 ELAPSED TlME{tiours) Figure 3. The changes in P^^ activity of the integument and viscera of H. atra incubated in filtered sea water enriched with phosphorus, as a function of incubation time. Each point represents the mean from three animals exposed for the same period of time while the vertical lines represent the ranges for each set of values. The P^^ activity of the integument from ani- mals previously treated with antibiotics is shown for comparison with the activity of the integu- ment and viscera under non-sterile conditions. Each point represented in the antibiotic curve indicates analyses from single animals sacrificed at the times shown. Millipore Filter, //,) and adding 100 /x-grams streptomycin and 100 units peni- cillin per ml. The animals were placed for two 90-minvite periods in this antibiotic solution. During the 12-hour interim between treatments, the animals were re- tained in 10 liters of aerated Millipore-filtered sea water. Following the second pre-incubation, each animal was placed in an individual receptacle containing 3 liters of filtered sea water to which P^^ bound as phosphoric acid was added. Ani-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfie
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