. Bacteriology in medicine and surgery. A practical manual for physicians, health officers, and students. Bacteriology. 234 BACTERIOLOGY. the bacteria deep down to near the bottom of the tubes while the media are still semi-solid. An anaerobic growth will take place in the lower part of the tube. In a similar way the closed arm of the fermentation tube will suffice for anaerobic growth, if the opening connecting it with the open bulb is quite small. In the more complicated methods the plates or tubes are placed in jars (Fig. 34), in which the oxygen is dis- FiQ. 34. Jar for anaerobic cultures.


. Bacteriology in medicine and surgery. A practical manual for physicians, health officers, and students. Bacteriology. 234 BACTERIOLOGY. the bacteria deep down to near the bottom of the tubes while the media are still semi-solid. An anaerobic growth will take place in the lower part of the tube. In a similar way the closed arm of the fermentation tube will suffice for anaerobic growth, if the opening connecting it with the open bulb is quite small. In the more complicated methods the plates or tubes are placed in jars (Fig. 34), in which the oxygen is dis- FiQ. 34. Jar for anaerobic cultures. placed by a stream of hydrogen developed by the Kipp apparatus through the action of pure granulated zinc and a pure 25 per cent, solution of sulphuric acid. When all the oxygen has been displaced the jars are sealed by rotating the stopper. In another method the oxygen is extracted by a mixture of pyrogallic acid and caustic potash. To each lOO of air space in the jar 1 gramme of pyrogallic acid and 10 of 6 per cent, solution of potassium hydroxide are added and the jars immediately sealed. When spores are present, a simple method suggested, I believe, by McFarland can be successfully employed. Vessels plugged with stoppers. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Park, William Hallock, 1863-1939; Guerard, Arthur Rose, 1851-. New York and Philadelphia, Lea Brothers & Co


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Keywords: ., bookcentury1800, bookdecade1890, bookpub, booksubjectbacteriology