Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . tures.) The addition of the white of an eggwill often clear it up; if this availsnot, refiltering several times and at-tention to the few points mentionedwill produce a clear Litmus Agar.—One per cent,lactose added to nutrient agar justbefore sterilization. Reaction neu-tral. One per cent, azolitmin(Kahlbaum) boiled five minutes andadded either to the tube before finalsterilization or, if media used inplates, added at the time of of
Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . tures.) The addition of the white of an eggwill often clear it up; if this availsnot, refiltering several times and at-tention to the few points mentionedwill produce a clear Litmus Agar.—One per cent,lactose added to nutrient agar justbefore sterilization. Reaction neu-tral. One per cent, azolitmin(Kahlbaum) boiled five minutes andadded either to the tube before finalsterilization or, if media used inplates, added at the time of of Nutrient Blood-serum.—If the slaughterof the animal can be supervised, it were best to have the siteof the woiuid and the knife sterilized, and sterUe flasks () at hand to receive the blood directly as it flows. The blood is placed on ice forty-eight hours, and theserum is drawn out with sterile pipets into test-tubes, avoid-ing shaking of the jar. These are placed obliquely in anoven where the temperatiu-e can be controlled and main-tamed. (See Fig. 21.) Coagulation of Blood-serum.—The tubes of blood-seriun. -Flask toblood-se- NUTRIENT CULTUEE-MEDIA 73 having been placed in the thermostat, are kept at a temper-ature of 65° to 68° C. mi to. coagulation occurs; then removedand sterilized by fractional sterilization. Sterilization of Blood-serum.—^The tubes are placedthree to four days in incubator at 58° C, and those tubeswhich show any evidences of organic growth are discarded. If, now, at the end of a week, the serum remains sterile at
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