. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 432 R. W. ROOT sired concentration. The blood was then equilibrated in air and hamlk-d the same as in the other experiments. Calculation of pll of Blood.—In calculating the pH of fish blood the familiar Henderson-Hasselbalch equation was used (Henderson, 1908; Hasselbalch, 1917). A pK' factor of was employed for the blood at 20° C. This was derived by using the average pK' factor of that has been worked out for mammalian serum at 38° C. (using Bohr's, 1905, solubility coefficient for CO«) by a series of workers (Wa
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 432 R. W. ROOT sired concentration. The blood was then equilibrated in air and hamlk-d the same as in the other experiments. Calculation of pll of Blood.—In calculating the pH of fish blood the familiar Henderson-Hasselbalch equation was used (Henderson, 1908; Hasselbalch, 1917). A pK' factor of was employed for the blood at 20° C. This was derived by using the average pK' factor of that has been worked out for mammalian serum at 38° C. (using Bohr's, 1905, solubility coefficient for CO«) by a series of workers (Warburg, 1922; Cullen, Keeler, and Robinson, 1925; Van Slyke, Hastings, Mur- ray, and Sendroy, 1925; and Hastings, Sendroy and Van Slyke, 1928), and adding a temperature correction of for each degree below 100. 10 20 jo 40 so 60 ro ao 90 100 Pa m m. Kg FIG. 1. Oxygen dissociation curves for toadfish blood at 20° C. Curve 1 at 1 mm. carbon dioxide; curve 2 at 10 mm. carbon dioxide; and curve 3 at 25 mm. carbon dioxide tension. 38° C. (Hasselbalch, 1917; and Warburg, 1922). In addition a correc- tion of was added 1 it-cause whole blood was used instead of serum. The pK' factor for whole blood is slightly higher than that for serum (Warburg, 1922; Peters, Bulger, and Eisenman, 1923; and Van Slyke etal., 1925). In using the pK' factor in the following calculations of pH, it is recognized that there are many variables which enter into its composi-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
