. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 220 CHARLES E. LANE AND ELEANOR DODGE effect. The nematocyst suspension at this time (Fig. 1) was almost completely free of tentacular tissue fragments and contained approximately 55 million nemato- cysts per wet gram, very few of them discharged. Nematocysts ranged in size from to micra. They fell into two size groups: one with a mean diameter of micra made up 23% of the total sample. The remainder varied about a mean diameter of micra. The packed nematocysts were frozen and stored at -- 5° C. Nematocysts
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 220 CHARLES E. LANE AND ELEANOR DODGE effect. The nematocyst suspension at this time (Fig. 1) was almost completely free of tentacular tissue fragments and contained approximately 55 million nemato- cysts per wet gram, very few of them discharged. Nematocysts ranged in size from to micra. They fell into two size groups: one with a mean diameter of micra made up 23% of the total sample. The remainder varied about a mean diameter of micra. The packed nematocysts were frozen and stored at -- 5° C. Nematocysts were still reactive after 20 weeks of frozen storage. An initial sample of liters of isolated fishing tentacles yielded 60 grams of packed wet "purified" FIGURE 1. Photomicrograph of isolated, purified, still-reactive nematocysts of Physalia. X 1400. The contents of the isolated nematocysts were liberated by homogenization in a chilled Potter-Elvejhem homogenizer. Amphibian Ringer's, sea water or distilled water may be used as the diluent. The sample was examined microscopically at intervals and homogenization was continued until about 90% of the capsules were fragmented (Fig. 2). The homogenate wras centrifuged at 600 g for ten minutes to separate capsules and capsular fragments from the diluted capsular contents. The supernatant solution was cloudy, yellowish-white in color, and extremely toxic to crabs, fish and small mammals. Precautions must be taken to avoid exposure of skin to contamination by any mixture, wet or dry, which contains undischarged nematocysts. Nematocysts on laboratory surfaces or clothing retain their reactivity for at least two weeks as unpleasant reminders of previous careless-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
