Archive image from page 249 of Cytology (1961). Cytology cytology00wils Year: 1961 Focal plane of eyepiece Rear focal plane of objective Objective Microscope stage Condenser Figure 11-12. Diagram Illustrating the Optical Path of Deviated and Undeviated Light Through the Ordinary Light Microscope. transmitted by the object will tend to cancel each other out (destructive interference) and make the object darker than its surroundings (Figure 11-13). This is dark or positive phase-contrast (Figure 11-14 (a)). Bright or negative phase-contrast, in which the object appears brighter than its surrou

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Archive image from page 249 of Cytology (1961). Cytology cytology00wils Year: 1961 Focal plane of eyepiece Rear focal plane of objective Objective Microscope stage Condenser Figure 11-12. Diagram Illustrating the Optical Path of Deviated and Undeviated Light Through the Ordinary Light Microscope. transmitted by the object will tend to cancel each other out (destructive interference) and make the object darker than its surroundings (Figure 11-13). This is dark or positive phase-contrast (Figure 11-14 (a)). Bright or negative phase-contrast, in which the object appears brighter than its surroundings, is obtained by retarding the undeviated light by Va wavelength at the diffraction plate (conjugate area). Since the deviated light has already been retarded by V4 wavelength at the object, the 230 / CHAPTER 11

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