. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 314 L. H. CHAPPELL, C. ARME, AND C. P. READ from infected Tcncbrio molitor. Rats received a diet of Purina Laboratory Chow. At 10 days post-infection rats were killed and the intestines removed to Krebs- Ringer-tris solution (KRT) at pH 7A. Worms were flushed from the intestines with KRT and washed several times; they were then randomized into groups of five. Each group of five worms was preincubated in 10 ml of KRT for 30 minutes at 37° C and was then transferred to 4 ml of incubation medium for 2 minutes. Following incubat
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 314 L. H. CHAPPELL, C. ARME, AND C. P. READ from infected Tcncbrio molitor. Rats received a diet of Purina Laboratory Chow. At 10 days post-infection rats were killed and the intestines removed to Krebs- Ringer-tris solution (KRT) at pH 7A. Worms were flushed from the intestines with KRT and washed several times; they were then randomized into groups of five. Each group of five worms was preincubated in 10 ml of KRT for 30 minutes at 37° C and was then transferred to 4 ml of incubation medium for 2 minutes. Following incubation, worms were washed twice in KRT, dried on Whatman No. 5 paper and transferred to 2 ml of 70% ethanol. Worm lipid was extracted in ethanol for 24 hrs, after which worms were removed to a drying oven at 90° C. Worm dry weight was recorded after drying for 24 hours. Aliquots (1 ml) of ethanol extracts were pipetted on aluminum planchets ( in diameter) and ml of a 10 mM sodium bicarbonate solution added. Radioactivity was determined with a Nuclear Chicago gas flow counter. 1-6 1-2. 1234 mM pal mi ta t e FIGURE 1. The uptake of 14C-palmitate as a function of palmitate concentration. Curve a is uptake of palmitate diluted from a 2 mM stock solution and Curve b is uptake of palmitate diluted from a 10 mM stock solution. Each point is the mean of at least 8 replicates. Uptake is expressed in /tMoles/g/2 min. Unless otherwise stated, all incubation media contained sodium desoxycholate (I/* Mole/ml). Radioactive palmitic acid, labeled with 14C at the carbon-1 position, was obtained from Nuclear Chicago Corps. The specific activity was /xC/mg. Unlabeled sodium palmitate (A grade) was obtained from K & K Laboratories. "A" grade stearic, oleic, linoleic, and linolenic acids, and sodium taurocholate were obtained from Calbiochem. Chrornatographically pure pentadecanoic, ara- chidic, and lignoceric acids were obtained from Sigma Chemical Co. and sodium desoxy
Size: 2281px × 1096px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
