. Biophysical science. Biophysics. 248 Structure of Viruses /14 : 2 many years. Present knowledge indicates that they may be atypical viruses (and, hence, poor standards). Nonetheless, they have been studied so widely that most of the material in this chapter is based on experiments with T phages of E. coli. Figure 1 shows a T phage attached to a bacterial Figure I. Electron micrograph of T2 phage particles attached to ghosts of E. coli B. Note that many of the phage particles are attached to the bacterial ghosts by their tails. After T. F. Anderson, American Naturalist 86: 91 (1952)


. Biophysical science. Biophysics. 248 Structure of Viruses /14 : 2 many years. Present knowledge indicates that they may be atypical viruses (and, hence, poor standards). Nonetheless, they have been studied so widely that most of the material in this chapter is based on experiments with T phages of E. coli. Figure 1 shows a T phage attached to a bacterial Figure I. Electron micrograph of T2 phage particles attached to ghosts of E. coli B. Note that many of the phage particles are attached to the bacterial ghosts by their tails. After T. F. Anderson, American Naturalist 86: 91 (1952). 2. Phage Studies Using Bacteriological Methods The routine method for analyzing bacteriophages involves bacterial plating techniques. To aid in understanding these techniques, the standard assay for determining bacterial concentration is first described. In this method, a large number of Petri dishes are made up with a sterile gelatinous medium on which the bacteria can grow. Each dish is carefully sterilized. One ml of the suspension of bacteria is poured. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ackerman, Eugene, 1920-. Englewood Cliffs, N. J. , Prentice-Hall


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