. Carnegie Institution of Washington publication. 228 BACTERIA IN RELATION TO PLANT DISEASES. turgid. (The third down was wanting except the base of the petiole.) The next morning all the tiny leaves at the tip (bevond the second leaf up) had collapsed. By 5 of the same day the wilted leaves had begun to shrivel. The fourth down was still turgid. The fourteenth day the fourth leaf had lost most of its turgor. The petiole of the pricked leaf was still green but it was flabby nearly to the base. The blades of the first and second leaves below had shriveled, also those of all the leaves abov
. Carnegie Institution of Washington publication. 228 BACTERIA IN RELATION TO PLANT DISEASES. turgid. (The third down was wanting except the base of the petiole.) The next morning all the tiny leaves at the tip (bevond the second leaf up) had collapsed. By 5 of the same day the wilted leaves had begun to shrivel. The fourth down was still turgid. The fourteenth day the fourth leaf had lost most of its turgor. The petiole of the pricked leaf was still green but it was flabby nearly to the base. The blades of the first and second leaves below had shriveled, also those of all the leaves above the pricked one. The upper part of the vine was now removed for examination and cultures. The vessels were found to be full of the bacillus which strung out in fine gummy threads from the cut surface of the stem when a needle-tip was touched to it and withdrawn. The bacteria were exceedinglv abundant and the inner tissues were considerably broken down. The organism was cul- tivated from this portion of the vine at different heights, inoculations being made from the stem into potato-broth from which a pure culture of Bacillus trackeiphilus was subsequently obtained on steamed potatoes. The fifteenth day the fifth leaf down was flabby. The twenty-ninth day this plant was removed together with the other old cucumber vines, to make room for squashes. Dry material was saved from this vine for the herbarium. A futile search was made in it for spores of the bacillus. (26.) The fifth leaf from the tip was pricked many times in one of the side lobes. At 10 , October 31 there were no signs but by ih 30'" of the same day the pricked lobe had wilted. The first signs appeared, therefore, in this case at the end of the sixth day, the inoculations having been made in the afternoon. By noon of the seventh day the wilt and change of color had made marked progress in the pricked lobe and that portion of the latter in which the wilt first appeared had dried out. The temperature in
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Keywords: ., bookauthorcarnegie, bookcentury1900, bookdecade1910, bookyear1911