. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CNS PEPTIDES FROM LIMULUS 153 of a typical G-50 experiment are shown in Figure 2. On this column LHGF eluted as a single symmetric peak with an estimated molecular weight of 6400. Active fractions from the G-50 runs were next treated with various proteolytic enzymes. Incubation of crude extracts with pepsin or protease resulted in a de- crease in LHGF activity of and , respectively, while incubation with trypsin had no effect on activity. Preliminary characterisation of LUC Several experiments were conducted on LU
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CNS PEPTIDES FROM LIMULUS 153 of a typical G-50 experiment are shown in Figure 2. On this column LHGF eluted as a single symmetric peak with an estimated molecular weight of 6400. Active fractions from the G-50 runs were next treated with various proteolytic enzymes. Incubation of crude extracts with pepsin or protease resulted in a de- crease in LHGF activity of and , respectively, while incubation with trypsin had no effect on activity. Preliminary characterisation of LUC Several experiments were conducted on LUC to determine its stability, suscepti- bility to various proteolytic enzymes, and molecular weight. As reported elsewhere (Brown and Cunningham, 1941 ), brief boiling of crude extracts had no effect on activity. However. LUC did appear to be susceptible to oxidation. The combined results of experiments conducted at both 20° C and 37° C showed that untreated extracts held for 20 hr lost of the original activity. In addition, treatment with thiodiglycol prevented this loss, while treatment with hydrogen peroxide lead to a loss in activity. In view of these results, all subsequent extracts were o oo 00 CO -z. LU O 2 0- I 0- o JZ 06 Q_ 0 04 ACTH (4600) Glucogon (3600). LUC (1850) l\ i I Kav Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
