. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. BACTERIOLOGICAL TECHNIC 83 with the tip of a heat-sterilized (by holding in flame of Bunsen burner until red hot) platinum needle (prepared by fusing a platinum wire, i3^ Inches long, into the end of a glass rod, six to seven inches long), then removing the cotton plug from the test-tube, and pushing the needle, carrying the microbes, into the culture medium down to the very bottom of the tube. Replace the cotton plug at once, pass the needle into the flame of the Bunsen burner until red hot, to sterilize it, and lay
. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. BACTERIOLOGICAL TECHNIC 83 with the tip of a heat-sterilized (by holding in flame of Bunsen burner until red hot) platinum needle (prepared by fusing a platinum wire, i3^ Inches long, into the end of a glass rod, six to seven inches long), then removing the cotton plug from the test-tube, and pushing the needle, carrying the microbes, into the culture medium down to the very bottom of the tube. Replace the cotton plug at once, pass the needle into the flame of the Bunsen burner until red hot, to sterilize it, and lay aside for the next tube inocu- lation. This is known as a deep stab tube inoculation. In this manner inoculate some five or six tubes. Also make streak inoculation on tube slants by simply passing the in- fected platinum needle over the middle of the tube slant surface, from lower end toward the top, ob- serving the instructions regarding the cotton plug and needle sterilization, with each tube inoculation. Number the tubes serially, and in a special note- book make entry of all desirable data pertaining to each inoculation, making such entries under each tube number. Place tubes vertically in a suitable holder, as tumbler, beaker, wire basket, etc., and set aside in incubator or in some container to which you alone have access. In warm weather the first bacterial growths may appear at the end of thirty-six hours. In cold or cool weather nothing may appear for two, three, and even four to five days. Note the nature of the bacterial growth in a deep stab inoculation and in the streak inoculation, as to a. Growth—scanty, moderate, abundant; slow, rapid. b. Form of growth—outUne clearly defined, spreading, rugose, beaded, etc. c. As to surface—flat, raised, concave, convex. d. Color—translucent, ghstening, waxy, trans- parent, opaque, fight, chalky white, grayish-white, dark red, green, blue, yellow, lemon color, purple, etc. e. Odor—comparative description. f. Consisten
Size: 1015px × 2462px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorschneide, bookcentury1900, bookdecade1920, bookyear1920
