. The Biological bulletin. Biology; Zoology; Marine biology. 336 MARGARET K. PETERSON AND JOHN BUCK Most of our material for electron microscopy was fixed in the field, and of necessity the fixation schedule was modified from the usual, since microscopes, hoods and temperature controls were not available. The abdomens of the specimens were slit open and the whole insects were dropped into 6% glutaraldehyde in Mil- lonig's buffer at ambient (tropical) temperatures. Some specimens were left in glutaraldehyde and others were transferred after an hour and a half to buffer con-. FlGURE 1. Figure 2.
. The Biological bulletin. Biology; Zoology; Marine biology. 336 MARGARET K. PETERSON AND JOHN BUCK Most of our material for electron microscopy was fixed in the field, and of necessity the fixation schedule was modified from the usual, since microscopes, hoods and temperature controls were not available. The abdomens of the specimens were slit open and the whole insects were dropped into 6% glutaraldehyde in Mil- lonig's buffer at ambient (tropical) temperatures. Some specimens were left in glutaraldehyde and others were transferred after an hour and a half to buffer con-. FlGURE 1. Figure 2. Figure 1. Semidiagrammatic drawing of a transverse section through the lantern of Pteroptyx, showing portions of the photogenic (PL) and dorsal (DL) layers and three cylinders (cyl) containing tracheal trunks (T). c, cortex of photocyte, pc; EO, end organ; pg, region of photocyte granules; pn, nucleus of photocyte; t, tracheole; tw, tracheal twig; cu, cuticle. Figure 2. Semidiagrammatic drawing of a horizontal section through part of the photo- genic layer of a Pteroptyx lantern, cyl, cylinder; T, tracheal trunk; tw, tracheal twig; t, tracheole; ten, tracheal epithelial nucleus; tn, tracheolar cell nucleus; EO, end organ; pc, photo- cyte ; c, cortex of photocyte; pg, region of photocyte granules; pn, photocyte nucleus. X 650. taining a small amount of thymol. They were in transit for from ten days to six weeks before reaching this laboratory, where they were post-fixed in Millonig's OsO^, dehydrated in alcohol, and embedded in Maraglas. Of these specimens, only Luciola showed satisfactory fixation. The other tissues were fixed poorly, but well enough to permit comparisons. Later, we were very fortunate in obtaining some live specimens of Pteroptyx. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Lillie, Fra
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