. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LEUCOPHORE-ACTIVATING SUBSTANCES 29 extract concentration of one-fifth of an eyestalk equivalent per dose. Every one of the 13 extracts was assayed on three eyestalkless prawns. The reason for assaying these extracts on three prawns whereas the previous extracts were assayed on five was that individual eyestalks extracted in only ml of fluid did not provide sufficient material for assays on a larger number of prawns because of small losses that occur when extracts are prepared and injected such as, for example, the small
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LEUCOPHORE-ACTIVATING SUBSTANCES 29 extract concentration of one-fifth of an eyestalk equivalent per dose. Every one of the 13 extracts was assayed on three eyestalkless prawns. The reason for assaying these extracts on three prawns whereas the previous extracts were assayed on five was that individual eyestalks extracted in only ml of fluid did not provide sufficient material for assays on a larger number of prawns because of small losses that occur when extracts are prepared and injected such as, for example, the small volume that always remains in the syringe and needle. The data for three of these 13 extracts are shown in Figure 2. The similarity of the data in Figures 1 and 2 justifies the use of the smaller number of prawns. The results were essentially the same as found with the extracts prepared from more than one eyestalk, some extracts produced dispersion alone, some concentration alone, and some a mixed response. With extracts of single eyestalks the largest responses showing pigment dispersion or concentration alone (Fig. 2) were less than those seen with the extracts prepared from several eyestalks (Fig. 1), reflecting the difference in extract concentration, one-fifth versus one-half of an eyestalk equiv- alent. The results for the entire 13 extracts showed that two produced dispersion alone, four concentration alone, and seven a mixed response. The original hypo- thesis that the mixed response was due to combining eyestalks that produced either. i - HOURS FIGURE 2. Relationships between the mean leucophore stage of eyestalkless prawns injected with extracts prepared from a single eyestalk versus time following injection. Symbols same as in Figure 1. Final extract concentration is: one-fifth eyestalk equivalent per Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
