. Dedication papers : scientific papers presented at the dedication of the laboratory building and plant houses, April 19-21, 1917. production whenthe mycelium is properly wounded. X 15. thus be obtained from a single Petri dish culture. Spores producedin this way were sprayed with an atomizer onto potato plants growingin a greenhouse. They produced good infection not only on the olderleaves but on young leaves as well. The method of stimulating spore production by wounding wastested out on each of the single spore strains isolated from the potato KUNKEL: SPORULATION IN MACROSPORIUM SOLANI 309
. Dedication papers : scientific papers presented at the dedication of the laboratory building and plant houses, April 19-21, 1917. production whenthe mycelium is properly wounded. X 15. thus be obtained from a single Petri dish culture. Spores producedin this way were sprayed with an atomizer onto potato plants growingin a greenhouse. They produced good infection not only on the olderleaves but on young leaves as well. The method of stimulating spore production by wounding wastested out on each of the single spore strains isolated from the potato KUNKEL: SPORULATION IN MACROSPORIUM SOLANI 309 fields of Maine. They all responded in like manner, producing sporesin great numbers. The method has also been applied to other Macro-sporiums that do not fruit readily in culture. M. tomato, Cooke, aparasite of the tomato and M. daturae, Fautr., a parasite of the jimsonweed, Datura Stramonium L. respond in the same way to the wound-stimulus. Although M. daturae fruits quite freely without thisstimulus, the number of spores produced in any culture can be greatlyincreased by wounding. M. tomato fruits even more sparingly than. Fig. 4. Fruiting culture of Macrosponum solani. This picture shows thespores more highly magnified and indicates their abundance in a wounded string-bean agar culture. X 250. M. solani in unwounded cultures. When thoroughly wounded itbears spores in great numbers. The abundant sporulation of M. solani in culture makes moreeasy the study of its fruiting stages. The successive steps in thedevelopment of conidiophores and spores can easily be mature conidiophores are always several-celled. They mayarise singly or in whorls, as is shown in Fig. 2. The typical conidio- 310 BROOKLYN BOTANIC GARDEN MEMOIRS phore bears a single spore. Successive production of spores on thesame conidiophore, such as has been described by Miyabe (7) forM. parasiticum Thiim., has not been observed for M. sola?ii. Occa-sionally the spores are borne in chains of two. This
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectplants, bookyear1918
