. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ABSORPTION SPECTRA OF HEMOCYANIN 153 in the hemocyanin. The union appears to depend upon some grouping in the hemocyanin molecule, of which the copper forms an essential part. For convenience we will refer to this arrangement as the " chromatic ; For purposes of comparison it is interesting to determine the absorption of light in relation to the number of chromatic groups present. According to Beer's Law, the extinction coefficient of a substance in solution is proportional to its concentration. We have conse
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ABSORPTION SPECTRA OF HEMOCYANIN 153 in the hemocyanin. The union appears to depend upon some grouping in the hemocyanin molecule, of which the copper forms an essential part. For convenience we will refer to this arrangement as the " chromatic ; For purposes of comparison it is interesting to determine the absorption of light in relation to the number of chromatic groups present. According to Beer's Law, the extinction coefficient of a substance in solution is proportional to its concentration. We have consequently expressed the absorption of light by the hemocyanin so- lutions in terms of E/c, where c is the concentration of copper in the solution expressed as milligram atoms per liter. An advantage of this notation also lies in the fact that the concentration of copper in serum may be readily obtained without the necessity of determining the number of grams of hemocyanin which are present, an investigation which cannot be made unless the hemocyanin of the species has been isolated and properly studied. THE APPLICATION OF BEER'S LAW TO HEMOCYANIN SOLUTIONS The foregoing treatment assumes explicitly that in the absorption of light by hemocyanin solutions Beer's Law is valid and that in con- sequence E/c is a constant characteristic of the substance at each wave /.o OB. I S 3 CONCEN TffA T/ON FIG. 1. Extinction coefficient of purified hemocyanin solutions of various concentrations. A. Busycon canaliculatum at 570 mju. B. Limulus polyphemus at 580 m/u. C. Limuhis polyphemus at 480 m/u. length. Quagliariello (1922) and Svedberg and Heyroth (1929) both present evidence that Beer's Law does not apply in the case of hemo- cyanin solutions. We have consequently examined this question care-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
