. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 59° Typhoid Fever Morphology.—The typhoid bacillus measures about i to 3 /^i (2 to 4 ju—Chantemesse, Widal) in length and to /* in breadth (Sternberg). The ends are rounded, and it is exceptional for the bacilli to be united in chains. The size and morphology vary with the nature of the culture-medium and the age of the culture. Thoi- not and Masselin,* in describing these morphologic variations, point out that when grown in bouillon the typhoid
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 59° Typhoid Fever Morphology.—The typhoid bacillus measures about i to 3 /^i (2 to 4 ju—Chantemesse, Widal) in length and to /* in breadth (Sternberg). The ends are rounded, and it is exceptional for the bacilli to be united in chains. The size and morphology vary with the nature of the culture-medium and the age of the culture. Thoi- not and Masselin,* in describing these morphologic variations, point out that when grown in bouillon the typhoid bacillus is very slender; in milk it is stouter; upon agar-agar and potato it is thick and short; and in old gelatin cultures it forms long filaments. It produces no spores. Flagella.—The organisms are actively motile and are provided with numerous flagella, which arise from all parts of the bacillus (peritricha), and are 10 to 20 in number. They stain well by. Fig. 248.—Bacillus typhosus. LofBer's method. The movements of the short bacilli are oscillating; those of the longer bacilli, serpentine and undulating. Staining;—The organism stains quite well by the ordinary methods, but not by Gram's method. As it gives up its color in the presence of almost any solvent, it is difficult to stain in tissue. When sections of tissue are to be stained for the demonstration of the tjrphoid bacilli, the best method is to allow them to remain in Loffler's alkaline methylene blue for from fifteen minutes to twenty- four hours, then wash in water, dehydrate rapidly in alcohol, clear up in xylol, and mount in Canada balsam. Ziehl's method also gives good results: The sections are stained for fifteen minutes in a solution of distilled water, 100, fuchsin i, and phenol 5. After staining they are washed in distilled water containing i per acetic acid, dehydrated in alcohol, cleared, and mounted. In such * "Pr6cis de Microbie," Paris, Please note that these imag
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1916
