. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHAETOPTERUS CENTRAL BODIES Fixation and Slide-making 151 The picro-acetic reagent employed in the experiments was made according to Boveri's formula (1887, p. 11) : 99 parts of a saturated solution of picric acid which has been diluted with two volumes of water, and one part of glacial acetic acid. In experiments where this formula was modified the details are given in each case. Unless other- wise stated, eggs were run up in the usual manner, sectioned at a thickness of 5 p, and stained in Heidenhain's hsematoxylin. Making
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHAETOPTERUS CENTRAL BODIES Fixation and Slide-making 151 The picro-acetic reagent employed in the experiments was made according to Boveri's formula (1887, p. 11) : 99 parts of a saturated solution of picric acid which has been diluted with two volumes of water, and one part of glacial acetic acid. In experiments where this formula was modified the details are given in each case. Unless other- wise stated, eggs were run up in the usual manner, sectioned at a thickness of 5 p, and stained in Heidenhain's hsematoxylin. Making Observations It is well known that the two asters of the first-cleavage figure in Chactoptents eggs differ in size (Fig. 1 ). In this paper only the larger aster of each figure was studied, in order to exclude possible variations due to differences in astral FIG. 1. The metaphase first-cleavage figure in Chactoftcrns eggs after using Boveri's picro-actetic fixation. The size difference between the two asters is apparent. The area outlined by the dotted lines is that part of the figure illustrated in the charts. There is here shown that type of structure occurring most frequently after using Boveri's picro- acetic fixation. Metaphase is here regarded as the time when chromosomes are aligned in a flat plate. Eggs in late prophase, when the chromosomes are in a broad irregular group, and in early anaphase, when they are just beginning to separate, were Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
Size: 1589px × 1572px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
