. The microscope; an introduction to microscopic methods and to histology. Microscopes. CH. VII~\ PAR AFFIX SECTIONING 1S5 It is hastened by warmth. It is then removed from the cedar-wood oil, drained, and placed in pure, melted paraffin, and this is then put into a paraffin oven and left from 2 to 24 hours (see Ch. X). It is then imbedded for sectioning.* Paraffin for infiltrating has usually a somewhat lower melting point than that for imbedding. Equal parts of paraffin of 43 C. and 54 C, answer well. For imbedding, the paraffin must be of a melting point which will give good ribbons in the
. The microscope; an introduction to microscopic methods and to histology. Microscopes. CH. VII~\ PAR AFFIX SECTIONING 1S5 It is hastened by warmth. It is then removed from the cedar-wood oil, drained, and placed in pure, melted paraffin, and this is then put into a paraffin oven and left from 2 to 24 hours (see Ch. X). It is then imbedded for sectioning.* Paraffin for infiltrating has usually a somewhat lower melting point than that for imbedding. Equal parts of paraffin of 43 C. and 54 C, answer well. For imbedding, the paraffin must be of a melting point which will give good ribbons in the temperature of the room where the sectioning is to be done. In a room of 19 to 20 C. a mixture of 1 part 43 C. paraffin with two parts of 54 C. usually answers well. Fig. 155. Hot filter for paraffin, gelatin, balsam, etc. It is entirely surrounded by a water jacket. The water is heated by placing a burner under the projecting part H. The wire basket is to hold the strainer and allow a free flow of the filtered substance on all sides. There is a bail for suspending the filter, and the filtered sub- stance runs out through the narrowed part \ 287. Imbedding in Paraffin.—Make a small paper box, fill it nearly full of hot paraffin, place the box for a half minute on some cold water to make a thin solid layer on the bottom, then transfer the tissue to the box and arrange near one end so that the sections may be cut in the desired plane. Now pi ace the paper box on as cold water as possible so that the paraffin may cool quickly. It will be more homogeneous if cooled quickly, and will shrink tightly against the tissue and avoid air spaces. In imbedding two main things should be looked after : The paraffin should be hot so that it will thoroughly fuse with the paraffin in the tissue. The tissue should be kept from the bottom of the box, either by holding it up in the middle of the box with warmed forceps or a perforated section lifter while a stratum *Thickened cedar-wood oil li
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