. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 232 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. Figure 2. I 'iews of a living, dividing yeast f Saccharomyces cerevisiaej produced by (A) green fluorescent protein (7) bound to nuclear histories and (B) DIC* (C) Image in DIC of the pm spacing between rows of fruslrual pores of the diatom Amphipleura illuminated with green light*. Images of a spindle iintlergmni; antiphase in Xenopus cytoplasmic egg extracts: (D) DAPI stained chromosomes. IE) Rhodamine-tubulin labeled spindle and aster microliibules. and (F) phase
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 232 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. Figure 2. I 'iews of a living, dividing yeast f Saccharomyces cerevisiaej produced by (A) green fluorescent protein (7) bound to nuclear histories and (B) DIC* (C) Image in DIC of the pm spacing between rows of fruslrual pores of the diatom Amphipleura illuminated with green light*. Images of a spindle iintlergmni; antiphase in Xenopus cytoplasmic egg extracts: (D) DAPI stained chromosomes. IE) Rhodamine-tubulin labeled spindle and aster microliibules. and (F) phase contrast (Nikon 20X/NA = Fluar Phase 3 objective). Stereo pair images (F) of DAPI stained chromosomes generated from a stack of 05 ^m optical sections through a Xenopus spindle fixed in the extracts in mid-anaphase* (*. Nikon 60X/AU = Plan Apo DIC objective and NA = L4 condenser illumination lor DIC) shutters in the light paths, filter wheel positions, image size and acquisition from the CCD camera, and image storage into stacks identified by their mode of acquisition (DAPI. Xrhod. phase, DIC, etc.). Image stacks are initially stored within the 64 MBytes of RAM memory, then archived on the hard drive or the Pinnacle optical disk drive. MetaMorph also provides comprehensive functions for quantitative analysis of intensity and motion, multi- color overlays of different image stacks (, DAPI and rhoda- mine channels), and movie presentation, either on the high res- olution graphics screen or by conversion of the digital images to video through a VGA to S-VHS video converter. Large stacks of images can also be converted to video for storage on an optical memory video disk recorder (Panasonic TQ2028 or TQ3038F). Using this system we were able to obtain novel high resolution real-time images of yeast nuclear motion in the cell division cycle (Fig. 2A, B) and to finally visualize in real time anaphase spindle dynamics and chromosome segregation in an in vitro system (3, 4)
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
