. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. 288 ELECTRON TRANSPORT. 20 30 40 50 60 TIME (MINUTES) 70 80 Fig. 8. Effectof light intensity on NAD (DPN) reduction by R. mbntm chroma- tophores. Reduction of NAD was followed by measuring the absorbancy increase at 340 m^. Reactions were carried out at 20°C in vacuum in Thunberg-type cuvette (1-cm. optical path). The standard components of the reaction mixture were as follows: M Tris containing 10% sucrose (Tris-sucrose buffer) of pH , ml.; M MgCl2, ml,; x 10-4 m NAD, ml.; R. nibriim chromatophor
. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. 288 ELECTRON TRANSPORT. 20 30 40 50 60 TIME (MINUTES) 70 80 Fig. 8. Effectof light intensity on NAD (DPN) reduction by R. mbntm chroma- tophores. Reduction of NAD was followed by measuring the absorbancy increase at 340 m^. Reactions were carried out at 20°C in vacuum in Thunberg-type cuvette (1-cm. optical path). The standard components of the reaction mixture were as follows: M Tris containing 10% sucrose (Tris-sucrose buffer) of pH , ml.; M MgCl2, ml,; x 10-4 m NAD, ml.; R. nibriim chromatophores (S) (A339 m/i/"^^'» approx. 50), ml. The total volume of cuvette was adjusted to ml. with water, and in the side chamber was M succinate (pH 8), ml. succinate/fumerate in darkness, so that when the E^ value of the oxidant is V or more negative, electrons do not flow from NADH to fuma- rate. If the E^ value is more positive, it may exceed the capacity of the regulatory mechanism, possibly for phosphorylation. Using cell-free extracts from Rhodopseudomonas palustris, Katoh (58) has found that oxygen uptake with succinate in darkness and in light was remarkably inhibited by ADP (half inhibition at x 10"^ M). In contrast, with the use of R. nibnim it was recently found that the light-stimulated oxidation of cytochrome C9 by chromatophores was markedly accelerated by the addition of ADP, and co a lesser extent by the addition of Pi (Fig. 10) (Horio and Yamashita, un- published). The addition of both ADP and Pi further accelerated the oxidation, which was not influenced by antimycin A. Duysens (59) and Goedheer (60) have reported that bacteriochloro- phyll is oxidatively bleached in light. Using ferri-ferrocyanide, the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Sy
Size: 2019px × 1238px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookcollectionbiodiversity, bookleafnumber312
