. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MUSSEL GILL SULFIDE OXIDATION 425 200 400 600 800 1000. 200 400 600 800 1000 0 200 400 600 800 1000 Na2S, Figure 1. Oxygen consumption rate («O:) of excised mussel gills as a function of sulfide concentration, in the presence (open symbols) and absence (closed symbols) of serotonin. Rates are given as mean ± SD. (A) Gills of freshly collected Geukensia demissa (circles); gills of mudtank-maintained G. demissa (squares); boiled G demissu gills (diamonds). (B) Gills of sulfide-free G. demissa (C) Gills of Mylilus galloprovinci
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MUSSEL GILL SULFIDE OXIDATION 425 200 400 600 800 1000. 200 400 600 800 1000 0 200 400 600 800 1000 Na2S, Figure 1. Oxygen consumption rate («O:) of excised mussel gills as a function of sulfide concentration, in the presence (open symbols) and absence (closed symbols) of serotonin. Rates are given as mean ± SD. (A) Gills of freshly collected Geukensia demissa (circles); gills of mudtank-maintained G. demissa (squares); boiled G demissu gills (diamonds). (B) Gills of sulfide-free G. demissa (C) Gills of Mylilus galloprovincialis. to sulfide slightly but significantly increased nO2, with a maximum near 20 pmol O; s"1 mg ' (n = 5) observed at 100-200 tiM sulfide (P 100 M/ sulfide (P < for 100 //A/ and 200 fiM sulfide; ANOVA), and at 1000 //A/sulfide were ± pmol O: s"1 mg~' (/; = 5; Fig. 1C), 38% of the serotonin- stimulated control value. Effect of cyanide on sulfide-stimulated oxygen consumption rate by gills of mudtank-maintained Geukensia demissa To test for mitochondria! participation in sulfide-stim- ulated nO2, the effect of cyanide, an inhibitor of mito- chondria! cytochrome oxidase, was determined in gills of mudtank-maintained G. demissa (Fig. 2). Control /;O: of these gills (see Fig. 1A) was inhibited by more than 90% in the presence of 1 mA/potassium cyanide (Fig. 2). Oxygen consumption rate in the presence of 200 mA/ sulfide (see Fig. 1A) was inhibited by cyanide to ± pmol O: s~' mg ' (n = 5; Fig. 2), representing a 61% inhibition of the rate in the absence of cyanide. Effects of sulfide on the oxidation/reduction state of cytochromes c and oxidase in mussel gills Difference spectra of G. demissa gills exposed to so- dium dithionite (1 mg mP') minus the same gills equili- brated with air showed reduced minus oxidized mito- chondria! cytochromes/i and c'and oxidase (Fig. 3, upper line). Under normoxic conditions, addition of sulfide at 20-200 nAI did
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