. Aquatic surveys and assessment of the Slim Buttes region of Harding and Butte Co., SD . Aquatic invertebrates; Aquatic invertebrates; Stream ecology; Stream ecology; Watershed ecology; Watershed ecology. Analysis of the sampled fish communities used Integrated Biotic Indices (IBI) (Bramblett et al. 2005) and derived Observed/Expected (O/E) Fish Models (Stagliano 2005) to detect impairment in the biological integrity of the sites. The IBI involved calculation of a series of metrics evaluating different attributes of the community (Table 2). The metrics allowed calculation of an overall score
. Aquatic surveys and assessment of the Slim Buttes region of Harding and Butte Co., SD . Aquatic invertebrates; Aquatic invertebrates; Stream ecology; Stream ecology; Watershed ecology; Watershed ecology. Analysis of the sampled fish communities used Integrated Biotic Indices (IBI) (Bramblett et al. 2005) and derived Observed/Expected (O/E) Fish Models (Stagliano 2005) to detect impairment in the biological integrity of the sites. The IBI involved calculation of a series of metrics evaluating different attributes of the community (Table 2). The metrics allowed calculation of an overall score between 0 and 100. Bramblett et al. (2005) did not propose threshold criteria for good, fair, and poor biological integrity for these scores. Therefore, we applied commonly used criteria. Scores of 75 to 100 indicate good to excellent biological integrity, 50-74 fair to good biological integrity, 25 to 49 indicated poor to fair biological integrity and scores <25% indicate poor biological integrity or severely impaired. Macroinvertebrate Communities Macroinvertebrates were collected from all habitats that we could wade within the sampling reach of the streams (Figure 8). Sampling involved multi-habitat, qualitative methods outlined in the EPA protocols (Barbour et al. 1999). Cobble substrates, vegetation, woody debris and bank-side areas were sampled qualitatively with a 500-micron D-frame net within the designated transects. A total of twenty habitat-weighted, randomized jabs or kicks were conducted within the reach, allowing 10 seconds per kick and composited into one sample. All organisms in the net were washed on a 500-micron sieve, transferred to a 1 liter Nalgene bottle, labeled and preserved in 95%. Figure 8. Multi-habitat macroinvertebrate sampling using EPA protocols. ethanol and brought to the MTNHP lab in Helena for processing. These samples were processed (sorting, identification, and data analysis) by David Stagliano at the Helena lab following DEQ protocol
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