A manual of the modern theory and technique of surgical asepsis . ered the con-sequence of infection. As shown before, it isnecessary to distinguish between real sapro-phytes and microbes xar e^o^r—that is, be-tween septogenic and pathogenic organisms—although this distinction is only relative. Whilesaprophytes which will settle on wound-surfacesand in cavities, as in the necrotic endometrium ofa puerperal uterus or in the intestines, originateptomaines, the absorption of which would be fol-lowed by toxic effects upon the living organism, INFLUENCE OF MICROBES. 29 the pathogenic microbes find
A manual of the modern theory and technique of surgical asepsis . ered the con-sequence of infection. As shown before, it isnecessary to distinguish between real sapro-phytes and microbes xar e^o^r—that is, be-tween septogenic and pathogenic organisms—although this distinction is only relative. Whilesaprophytes which will settle on wound-surfacesand in cavities, as in the necrotic endometrium ofa puerperal uterus or in the intestines, originateptomaines, the absorption of which would be fol-lowed by toxic effects upon the living organism, INFLUENCE OF MICROBES. 29 the pathogenic microbes find the most favorableconditions for their development and multiplica-tion in living tissue—that is, inside of cells, in theblood, in the lymphatics, etc. By thus invadingthe system they cause a series of pathogenic microbes differ essentially fromthe decomposing micro-organisms, and are killedby them in dead tissue. Hence decomposition,strange to say, is itself the most effective anti-parasitic agent to overcome the action of Fig. 9.—Staining the bacillus tuberculosis. No scientific method of isolating and cultivat-ing a distinct species from a mixture of thesedifferent microbes was known until Robert Kochdiscovered the mode of disseminating a mixtureof them over a large surface in order to favorthe development of the various species. Thenthe different forms of vegetation could be recog-nized with the naked eye as spots or turfs of apeculiar shape, color, growing species, etc. On an 3° SURGICAL ASEPSIS. artificially prepared soil, especially on gelatin oron agar, pure cultures can readily be of them are characterized by their capacityto liquefy the gelatin, while others form whitedry heaps or white mucilaginous drops, or formcolonies of a yellow, green, or red color. A very important aid in distinguishing thesedifferent forms under the microscope is theirstaining (Fig. 9) with certain coloring matters, es-pecially aniline dy
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectsurgery, bookyear1895
